Cell lysates were clarified by centrifugation at 36200 g for 30 min, and the supernatant was filtered using a 0.22 μm membrane (Corning filter system) prior to protein purification.
First, the RNA later® soaked membrane of the filter system allows flexibility in handling time as the RNA is stable for 24 hours at 37 °C, one week at 25 °C and one month or more at 4 °C.
All solutions used in a high-performance liquid crystal (HPLC, Waters Associates, Milford, MA, USA) analysis were filtered and degassed using a 0.22-μm membrane filter with a filtration system.
The microbial load of effluents after conventional treatment is very high, as microbial sludge settle through secondary clarifier under gravity, while in case of MBR-based treatment systems, membrane filter is used to separate sludge from the water.
The membrane filter (Skyhydrant™ water filtration system, Skyjuice ™ Foundation Inc ,ustrAustralia a commercially available, gravity-fed, source based waser filtration system that has previously been pilot-tested on the residential commerciallyristiavailablel College (CMC), Vellore and demonstrated potential as a low-cost solution for water disinfection in smaller communities [ 32].
The clear fraction was filtered through a 0.2 μm nylon membrane filter and injected onto HPLC system (Alliance 2690/5: Waters Corporation, Milford, CA), equipped with a 4.6 mm × 150 mm Agilent Zorbax Carbohydrate Analysis column (Agilent Technologies Inc., USA).
They were seeded (day 0) at 1.0 × 10 or 4.0 × 10 cells/mL onto a dish or a membrane filter of an insert culture system (polyethylene terephthalate, 0.4 μm high-density pores, and 0.3 cm, BD Falcon, Franklin Lakes, NJ, USA), respectively.
The solutions were filtered through a 0.45μm membrane filter before injection into the HPLC system.
All solutions were filtered through a 0.45 µm membrane filter before injection to the HPLC system.
Then, the filtrate were stored at 4 °C and filtered through a 0.22 μm membrane filter before injection into the LC system for analysis.
