A pure plasma membrane protein extraction method, as well as normalized TRPV4 expression using pan-cadherin as loading control for densitometry, was utilized to ensure the accuracy of the observation.
This article reviewed common techniques including membrane technology, centrifugation, extraction methods, chromatographic methods, spectroscopic methods, electrochemical methods and calorimetry.
If you just want to get the binding affinity of the active herbal components and plasma proteins, membrane technology, centrifugation, extraction methods and chromatographic methods are the good choice.
Phospholipids were separated from membranes by Müller's acidic extraction method and analysed in thin-layer two-dimensional chromatography.
DNA extraction from fluoropore membranes was performed with the FastDNA extraction method (Qbiogene, Irvine, CA, USA), by using a procedure previously published (8 ), and PCR was performed on extracted DNA by using primers Toxo B22 and B23 (9 ).
In order to confirm our results, LMW-GS in B. distachyon were extracted with a wheat glutenin extraction method and separated by SDS-PAGE, and then transferred to PVDF membrane for Western-blotting and antibody-based identification.
The TCA-based extraction method was developed to solubilise integral membrane proteins and thus extracts proteins efficiently from yeast cells [5].
In the present study, a simple and sensitive extraction method based on polypropylene membrane-protected micro-solid phase extraction (MP-μ-SPE) has been developed for analysis of sulfonamides in food samples.
Cell membrane extraction was carried out according to the methods reported previously [ 8].
Acomparison of the traditional biotin-streptavidin system with the sucrose gradient-based extraction kit shows that the sucrose gradient-based extraction method yields a highly enriched, pure, plasma membrane fraction, while the biotin-streptavidin method produces a crude plasma membrane fraction with considerable contamination of cytoskeletal proteins.
