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Partially-rounded cells with one or two membrane extension processes were assigned half-a-point.
Changes in cell morphology were scored manually employing the following criteria: Cells that had a fully-rounded cell body with no membrane extension processes were given one point.
Non-rounded cells having three or four membrane extension processes and with a cell morphology similar to wildtype HEK-293 cells, were given zero points.
Similar(57)
The ability to recruit Dia, one of the central nucleators of actin microfilaments, to the cell membrane, is important for its capacity to trigger processes of membrane extension and intracellular trafficking.
Our findings highlight the importance of the FRMD7 CASK interaction in promoting membrane extension during neurite outgrowth.
Therefore, this differentiation process is expected to consist of latent and extension processes.
The actin cytoskeleton generates forces required for membrane extension and remodelling, motor protein-dependent cell contraction or membrane trafficking [ 1].
These 'metastable' cells present both residual E-cadherin and collective migration, membrane extension [ 76].
Microtubule phenotype: processes, long narrow membrane extensions containing microtubules.
Cell migration is a multi-step process requiring new membrane extensions at the leading edge, the formation of new adhesions to the substrate, and contractions at the trailing edge to move cell body forward (Ridley et al., 2003).
Figure 2 The array aperture extension process.
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