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No induction of Fas membrane expression was observed in the mutant p53-expressing NCCIT after the various treatments (Supplementary Figure 3b).
Induction of Fas membrane expression was not detected in the mutant p53-expressing NCCIT cells after cisplatin or Nutlin-3 treatment (Supplementary Figure 3b).
Membrane expression of E-cadherin was observed after 1 day in 3D culture [22], whilst Dsg2 membrane expression was not seen until day 4 (Figure S2C).
Intense membrane expression was observed at cell-cell junctions with both EpEx and Ep-ICD domain specific antibodies (Figure 8 - A, D, F, and G).
Kv12.2 channel membrane expression was assessed by membrane surface biotinylation assay utilizing a 'Cell surface protein isolation kit' (Pierce, IL), according to instructions from the manufacturer.
To exclude non-specific staining, membrane expression was only measured in viable (7-AAD negative) cells which had intact plasma membranes.
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Extended Data Fig. 3 SDC1 membrane expression is elevated in KRAS*-driven PDAC.
BCMA membrane expression is universally detected in patient-derived CD138-positive myeloma cells, but not in other tissues.
Intestinal absorption and renal reabsorption of phosphate are mediated by members of the SLC34 family of sodium-coupled transporters (NaPi-IIa,b,c) whose membrane expression is regulated by various hormones, circulating proteins, and phosphate itself.
Decreased/lost ALCAM membrane expression is a marker of poorer outcome in epithelial ovarian cancer.
On the contrary, in breast [24] [26] and ovarian cancer [20] ALCAM cytoplasmic overexpression and low membrane expression were associated with disease progression.
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