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Accumulation of sterols in the ER membrane triggers binding of the membrane domain of HMGCoAR to a subset of Insigs, which carry a membrane-anchored ubiquitin ligase called GP78 which ubiquitinates HMGCoAR, marking it for proteasomal degradation [83].
This result supports the fact that the membrane domain of BmrA remains fully functional under these conditions.
One membrane domain of TCs faced Ciona intestinalis oocyte, whereas the other was closely associated with an extracellular matrix (chorion).
Sb was shown to promote the redistribution of the collagen receptor from the lateral to the basal membrane domain of enterocytes.
The finding that human mutations in CRB1 lead to retinitis pigmentosa [15] emphasizes the importance of deciphering the molecular networks associated with Crb in the apical membrane domain of the Drosophila photoreceptor.
In step 1, the full length HA gene of the pandemic 2009 A(H1N1) influenza virus (including the core HA protein of 519 aa, the signal peptide of 16 aa, and the inter membrane domain of 36 aa) was digested and re-assembled by PCR to form a pool of fragments enriched in 100 500 bp [24].
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The trans-membrane domain of nsp3 is sufficient to mediate ER membrane association of the cytosolic protein EGFP [24].
Membrane-bound isoforms were amplified using primers specific for exon 3, corresponding to the common trans-membrane domain of DC-SIGNR.
The deletion of exon 3 eliminates the trans-membrane domain of the protein and leads to the expression of soluble DC-SIGNR isoforms [3].
Generation of a sorting insensitive Robo was achieved through an in vitro assay and an unbiased approach that led to the mapping of the peri-membrane domain of Robo as the only Comm-interacting domains.
Amantadine and rimantadine target the M2 protein, and single mutations in the trans-membrane domain of M2 (e.g. residue 26 (L→F), 27 (V→A or T), 30 (A→T or V), 31 (S→N or R), and 34 (G→E)) can confer resistance to these drugs [38].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com