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It was reported that lipid peroxide was treated as key process to erythrocyte membrane disruption after exposure to DEP [16] and incubation of mouse erythrocytes with SiNPs (50 nm) caused significant increase in the MDA content [18], which were fairly consistent with our results of the MDA content (Fig. 5a).
Elevated LDH activities therefore reflected cell membrane disruption after sorafenib treatment.
In our experimental setting, apoptosis induction and membrane disruption after sorafenib treatment was not significantly influenced by the histological origin of and BRAF mutational status of thyroid carcinoma cells.
Presumably, intracellular calcium concentrations above 100 μM can be achieved only in situations of pronounced membrane disruption after serious cell damage or death, and not even during apoptosis [ 37, 38].
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Breast cancer cell-membrane disruption after NRC-03 and NRC-07 treatment was confirmed by propidium iodide uptake by MDA-MB-231 cells after 10-minute exposure to peptide (data not shown).
After membrane disruption, it binds to DNA by intercalating between the bases with little or no sequence preference.
Calcein release after membrane disruption was evaluated by measuring fluorescence intensity at 515 nm with excitation at 492 nm on a RF-5300PC spectrofluorometer at room temperature.
The extracellular conductivity in the P. digitatum suspensions with α-terpineol was higher than that of the control at all times, the values increased significantly after 30 min of exposure and positively correlated with the α-terpineol concentration (Fig. 2b), indicating rapid leakage of metal ions tested after the membrane disruption.
Repair following treatment with each of these lectins was then evaluated after creating a plasma membrane disruption with a laser.
During infection, bacteria trigger platelet activation via PF4 and TLRs and can initiate neutrophil extracellular traps (NETs) release by neutrophils after chromatin decondensation and nuclear membrane disruption.
To get further knowledge about the mechanisms of erythrocyte membrane disruption, we measured the MDA content and SOD activity after SiNP incubation at different concentrations.
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