Discontinuity of plasma membrane also occurs in some affected neurons (red arrows in Figs. 6C and S7) suggesting membrane destabilization likely due to an abnormality in intraneuronal homeostatic condition.
We hypothesized that this delivery strategy would facilitate improved transfection efficiency while eliminating the toxicity seen for many vectors due to membrane destabilization.
Polyplexes formulated with polymers containing tertiary amine (DMAEMA) charges were found to have higher cellular internalization profiles but were significantly more toxic to cells (due to membrane destabilization).
Spontaneous myelin breakdown, due to membrane destabilization caused by the incorporation of abnormal VLCFA into myelin-specific lipids has been suggested as a cause for the initiation of inflammation (33).
Compared to non-malignant cells, cancer cells often have a greater membrane fluidity [ 17, 18] and cell surface area (additional microvilli) [ 19, 20], hence leading to an improved anticancer activity of ACPs due to an increased membrane destabilization and the ability to bind more ACP molecules.
Moreover, the modification with aptamer and HA2 (GS-PEG/HA2-Apt) could enhance the cellular uptake by 15 73 % in comparison with GS-PEG-Apt, which is due to the fact that induced HA2 peptide mediates cell membrane destabilization, further enhancing endosomal escape and increasing cellular distribution [28].
The nucleotide leakage and outer membrane (OM) permeability assays were used to verify membrane destabilization.
The mechanism of disruption remains undefined, but albumin binds fatty acids46 and sterols47 and likely induces membrane destabilization.
Miyata, K. et al. Polyplexes from poly aspartamide) bearing 1,2-diaminoethane side chains induce pH-selective, endosomal membrane destabilization with amplified transfection and negligible cytotoxicity.
The purified polypeptide promoted the requisite steps of fusion, membrane destabilization, lipid mixing and aqueous contents mixing.
