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The results provide some support for our contention as indeed, the apical membrane content of the doubly perturbed cells approximates wild type.
Second, the GLUT4 immunoreactivity increased in plasma membrane lawns prepared from basal and insulin-stimulated adipocytes (Figure 8B), without any concomitant modification of the plasma membrane content of TfR (Figure 8C).
To complement our quantifications of apical cell area and gain some insight into the membrane content of the AS cells, we calculated the ratio between the real cell perimeter and the perimeter of the best-fitted ellipse (Figure 5A).
On the other hand, the overexpression of APT-2 caused a significant reduction in the plasma membrane content of GAP-43full at 3 h of 2-BP treatment (Fig. 5C).
Indeed, it was shown that the plasma membrane content of IRAP, which recycles like GLUT4 in adipocytes and has an unaltered expression, is increased in plasma membranes of adipocytes isolated from diabetic patients [4].
The susceptibility of bacteria to daptomycin is correlated with the membrane content of PG.
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Consistent with this interpretation, the Indess(k) showed a negative correlation with the membrane content independent of k (Additional file 2, Figure S5).
In addition, it gives an estimation of the excess of apical membrane content in AS cells upon Rab5DN expression and the decrease of membrane content in AS cells upon Rab11DN expression.
We determined whether CD36 deficiency alters ABCA1 membrane content by biotin labeling of macrophage surface proteins.
Free radical oxidation of the retinal photoreceptors adversely affects visual processing after the loss of lipoprotein membrane content [ 3, 4].
The antibacterial activity of daptomycin is calcium-dependent and correlates with the target membrane's content of phosphatidylglycerol (PG).
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