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Towards later stages, this excess in the membrane content is reduced and cells exhibit again straighter membranes (Figure 5D).
Along the same lines, if Rab11 function is impaired, the insertion of membrane through Rab11 endosomes will be affected, and the apical membrane content is reduced, as we observed (Figure 6H).
The figure also shows clearly that the gradients in initial chemical potentials are much larger for PIP2 than PS, although its membrane content is much smaller.
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High SCD1 protein and activity levels, leading to an increase in MUFA membrane content, are acknowledged to be critical features allowing a more fluid membrane organisation to be achieved (Stubbs and Smith, 1984; Scaglia et al, 2005).
When the Arabidopsis fatty acid deficient triple mutant fad3-2 fad7-2 fad8, whish is devoid of trienoic fatty acids, was exposed to prolonged low temperatures, a decrease in fluorescence parameters, chlorophyll content, and thylakoid membrane content was observed compared with wild-type plants [ 4].
Furthermore, in the absence of PDR18 the effect of 2,4-D in the plasma membrane sterol content is even more pronounced than in wild-type cells.
Membranes are main targets of degradative processes induced by drought and it has been shown that, under water stress, a decrease in membrane lipid content is correlated to the inhibition of lipid biosynthesis [ 72, 73] and a stimulation of lipolytic and peroxidative activities [ 74- 77].
Membrane liquid order, influenced by membrane cholesterol content, was investigated in HEK293T cells.
Reversely, fusion of one particular vesicle with the targeted membrane where their content is delivered to insure its function, leads to the dispersion or the active dissociation of many molecular species.
Silverman et al have also extensively studied exosome production by L. donovani and proposed that exosomes fuse with the plasma membrane and their content is released into the cytoplasm of the macrophage [11].
Contact angle measurement of membranes and water content is considered to be an important parameter for membrane characterization, since the pure water flux of the membrane can be predicted based on these results.
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