The formation of membrane clusters was quantified via fluorescence intensity vs time and changes in the temporal auto- and cross-correlations above a single nanoscale aperture.
Given that APC associates with the plus ends of microtubules, binds to other plus end-binding proteins, and its localization to cortical membrane clusters is microtubule-dependent [ 10, 21, 23- 27], we next determined whether protrusion formation and APC/β-catenin complexes required intact microtubules.
The mechanisms that segregate Nav channels in the AIS proximal (Nav1.1) or distal (Nav1.6) subcompartment, in addition to their AnkG-mediated membrane clustering, are currently unknown.
Despite its ubiquitous membrane localization, prepatterned AChR clusters were restricted to medial membranes and to the muscle center, identical to the wildtype situation (Figure 6C H).
Other enriched GO term clusters were membrane (7 genes) together with cell division, cell cycle and cytoplasm (9 genes).
For the 407 Cbr-GLD-1 targets common to bilaterians, significant GO term "functional annotation clusters" were intracellular membrane bound organelle and nucleus; reproductive process; anatomical structure morphogenesis; and multicellular organismal, embryonic, postembryonic, and larval development (Bonferroni-adjusted P values < 0.005, fold enrichment > 1.5, >15% input genes for each term).
These genes were grouped into 105 functional clusters (Additional file 2), among which the most significantly enriched clusters were related to membrane transporters (25 genes) and transmembrane proteins (146 genes) (Table 4).
CD8 Tregs suppress by releasing exosomes that carry preassembled NOX2 membrane clusters and are taken up by CD4 T cells.
Membrane areas further than 500 nm from the edge of clusters were defined as extrasynaptic.
This subunit has a non-uniform plasma membrane distribution; Kv2.1 clusters are frequently adjacent to, but never overlap with, GABAergic synapses.
