However, although it is known that Ply requires membrane cholesterol for its cytolytic effects, it has not been definitively shown that cholesterol functions as the cellular receptor.
Depletion of membrane cholesterol had a similar effect on syntaxin 4 dispersal, leading to non-polar exocytosis.
This is the first study on the effects of membrane cholesterol depletion in mRNA expression in myogenic cells.
Rather, agisterol appears to work through a mechanism that directly interferes with oxysterol effects on membrane cholesterol accessibility.
Here, we investigated the effects of membrane cholesterol depletion in the whole transcriptomic profile of chick skeletal muscle cells, using an Ion Torrent-based sequencing.
Thus, according to the distribution of HFAs versus NFAs in sphingolipids, an increase of membrane cholesterol can lead to opposite effects on sphingolipid-mediated amyloidogenic protein binding and aggregation.
In this study, we investigated whether COD purified from Bordetella species (COD-B) could suppress the growth of a lung adenocarcinoma cell line by its effect on membrane cholesterol.
We show that the ability of LXRs to activate transcription of the Abca1 sterol transporter, and thereby alter membrane cholesterol homeostasis, has a secondary effect on inflammatory signaling through inhibition of NF-κB and MAPK signaling pathways downstream of TLRs.
Using this PFO binding assay, we demonstrate that 25-HC increases the availability of membrane cholesterol, and that this cholesterol activating effect is specific to side-chain oxysterols.
One possibility is an effect on plasma membrane cholesterol because extraction of cholesterol with methyl-β-cyclodextrin also inhibits the formation of membrane ruffles at the plasma membrane, and inhibits the reorganization of filamentous actin at the cell periphery necessary for the formation of membrane ruffles [ 36].
