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Current densities, expressed per unit membrane capacitance, were measured from the current at −100 mV during the ramp protocols.
Series resistance and membrane capacitance were compensated prior to acquiring any recordings.
No significant differences in membrane capacitance were observed at any age.
Whole-cell currents were recorded at 20 kHz, pipette and membrane capacitance were compensated using Patchmaster macros and series resistance was compensated by 70%.
Pipette and membrane capacitance were compensated using the auto function of Patchmaster and series resistance was compensated by 70% to minimize voltage errors.
Membrane resistance, series resistance, and membrane capacitance were determined from current transients elicited by a 5mV depolarizing step from a holding potential of −80mV, using the whole-cell application of PATCHMASTER software.
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Cell membrane capacitance was measured by dividing the decay time constant of the capacitive transient in response to 5 mV depolarizing steps from −40 mV, by the Rs.
Cell membrane capacitance was estimated by dividing the decay time constant of the capacitive transient in response to 5-mV hyperpolarizing voltage clamp steps from 0 mV by the series resistance, and amounted to 34 ± 3 pF (mean ± SEM; n = 12).
(C_{m}), the neuronal membrane capacitance, is (21~mbox{pF}).
As a result, increase in the membrane capacitance is recorded.
The membrane capacitance was 50 100 picofarads.
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