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To assess the membrane blebbing potency of DRP-1β, DRP-1 and ZIPk, 293T cells were transfected with the appropriate plasmid and 1 µg of peGFP expression vector.
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The fluorescence spectroscopy, flow cytometry, and electron microscopy observation indicated that IR2 exhibited great membrane penetration potential by inducing membrane blebbing, disruption and lysis.
Atomic force microscopy revealed that KTP derivatives perturbed Staphylococcus aureus membrane structure by inducing membrane blebbing, disruption and lysis.
This is similar to that of cell necrosis, but necrosis is usually accompanied by cell membrane blebbing.
At 4-8 h post-infection, the cell membrane blebbing and apoptotic vesicles were observed and the nuclear membrane was broken apart.
a, b The white arrows indicate cells exhibiting the typical phenomena of apoptosis, with membrane blebbing, apoptotic membrane protrusions, and apoptosis bodies.
Plasma membrane blebbing and DNA fragmentation of peptide-treated tumor cells indicated that the peptides could induce apoptosis in tumor cells.
ROCK inhibition decreases nocodazole-induced membrane blebbing and stabilizes MTs.
Interestingly, the inactivation of another Rac effector, filamin, also induces prominent membrane blebbing [32].
FHOD1, another functional similar formin, contributes to plasma membrane blebbing and enhances invasiveness [48].
Instead a large fraction exhibited membrane blebbing as previously observed in regular tissue culture dish.
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