In wild-type cells, EGFP-WT-α-syn primarily localized to the plasma membrane (Fig. 5); whereas, in elo1Δ cells, EGFP-WT-α-syn accumulated in multiple cytosolic inclusions or vesicles per cell, and, in general, less plasma membrane binding occurred.
Located on the opposite side of the membrane where propeptide binding occurs, peptide 25 40 undergoes minor (1.0 2.0 Da) changes in deuterium incorporation at the 10 s and 1 min labeling time points.
Insig binding occurs through the membrane domain of reductase, which contains eight membrane-spanning helices and precedes a large cytosolic domain with enzymatic activity (Liscum et al., 1985; Roitelman et al., 1992).
Structural models and mutagenesis studies of TAAR1, TAAR7e, and TAAR7f predicted that ligand binding occurs in the membrane plane, Asp3.32 forms a salt bridge to the ligand amino group, and other transmembrane residues provide a scaffold that supports ligand binding and enables selectivity (Ferrero et al., 2012; Reese et al., 2014).
The highest degree of binding occurs in washed mitochondria, microsomes, ribosome-free microsomal membranes and their constituent core proteins.
We had previously demonstrated that extracellular binding occurs either by direct attachment of bacteria or yeast to the neutrophil surface or by binding of these cells at a distance through cytonemes (dynamic thread-like membrane tubulovesicular extensions of neutrophils [ 32]).
Our findings suggest that these polymers operate with a concentration-dependent mechanism of action: at low concentrations permeation and DNA binding occur without membrane disruption, while at high concentrations complete disruption of the membrane occurs.
The GAG binding occurred primarily in the outer 50 70 μm of the membranes, so that coupling was homogeneous only for thin gels.
The greatest level of IgG binding occurred with the EEV structural proteins F13L and A33R, and the IV membrane scaffold protein D13L.
Mitochondrial binding occurred preferentially over the vegetative 'meront' stages of the parasite, which bulged into the cytoplasm, thereby increasing the membrane surface available for mitochondrial interaction.
