Sentence examples for membrane binding method from inspiring English sources

Viral RNA was isolated using the QIAamp Viral RNA Kit (Qiagen) silica membrane binding method, and the protease and RT genes were amplified in seven different RT-PCR reactions.

We extracted genomic DNA from cryopreserved lymphocytes using a modified salt precipitation-extraction method (Gentra Systems, Minneapolis, MN) or from granulocytes using a silica membrane binding method (Qiagen Inc., Valencia, CA).

To further demonstrate the implication of a membrane binding site, we used a binding assay method based on the interaction of E-HRP as a specific probe to detect E2 binding sites at the plasma membrane of nonpermeabilized cells.

(A and B) Membrane binding co-sedimentation assays (See Materials and methods).

To test the predictions of the working model, we have employed single-molecule methods to analyze the membrane binding and surface diffusion of full-length PKCα and truncation constructs lacking one or more domains.

In this aim, we have developed a method for the identification of the testosterone membrane binding sites on formalin-fixed, paraffin-embedded routine prostate tissue sections, as described in Material and Methods.

The obtained HSA binding of known drug molecules were compared to the Immobilizd Artificial Membrane binding data (CHI IAM) obtained by our previously described HPLC‐based method.

Because PKC ligand binding was shown to be dependent on phospholipid binding [32], it is perhaps not surprising that our method failed to predict PKC-delta as a druggable site since we do not treat the effects of membrane binding.

Detailed methods for synthesis of precursor and reference compounds, the affinity testing of the new radiolabelled ligand via NET-expressing membrane binding protocol, as well as detailed procedures to autoradiography, immunohistochemistry and metabolic stability testings.

Internalization and membrane binding studies showed that 15.2% of the activity is bound to the membrane and 84.8% is internalised.

These lipoplexes carry a positive surface charge which aids internalisation through cell membrane binding.