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Samples were further used in experiments for membrane array analysis.
Membrane array analysis was performed on 10 human CRC tissue samples and 10 paired human normal colorectal tissue samples.
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At least three independent sets of array analysis using duplicate array membranes were performed.
To test the SMALI predictions, we carried out a peptide array analysis of 15-mer TSAd phosphotyrosine peptides spotted to a nitrocellulose membrane.
Subsequently, homogenates were centrifuged at 21 000 g for 15 min at 4°C to pellet membrane material, and supernatants were removed and stored at −70°C until ELISA and multi-plex cytokine microbead array analysis as described below.
Synthetic genetic array analysis identified genetic interactions of tra1- SRR3413 with genes involved in gene expression, mitochondrial function, and membrane sorting/protein trafficking (Hoke et al. 2008b).
Gene array analysis and expression of Notch pathway genes.
reverse phase protein array analysis.
MicroRNA array analysis was performed as follows.
In this study, we performed GeneChip analysis to identify multiple extracellular determinants, such as cytokines, cell membrane-bound molecules and matrix responsible for cardiomyogenic differentiation, and evaluated the statistical significance of differential gene expression by NIA array analysis (http://lgsun.grc.nia.nih.gov/ANOVA/) [20], a web-based tool for microarray data analysis.
The array analysis indicated that this region was intact in all isolates tested on array.
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