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Values in membrane and interior were summed for red and green channel, and also sizes were measured.
The additional formation of dysfunctional protein (desmin) aggregates in subplasma membrane and interior cellular compartments contributes to the deleterious phenotypes of plectin-deficient myofibers.
Values in membrane and interior were summed for both channel, also sizes were measured In order to compute the relative amount of internalized red fluorescence we estimated QD total (QD membrane +QD interior ) and calculated the ratio QD iinterior /QDtotal for each cell.
The extent of endocytosis was quantified after segmentation of each cell into membrane and interior, calculating the ratio between the signal from the cytosol (QDinterior) over the total (QDtotal = QDinterior + QDmembrane) (ratio: QDinterior/QDtotal) using image processing tools on individual cells at different time points (See Methods).
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With a mask marking red pixels (QD655) membrane was defined as the product mask × pre-membrane, and interior as the product mask × pre-interior.
With this mask a membrane was defined as the product of the QD mask and pre-membrane, and interior as the product of the QD mask and pre-interior obtained previously.
To compute the relative amount of internalized red fluorescence we estimated QD total as the sum of QD membrane and QD interior and we calculated for each cell the ratio QD interior /QD total.
These lipid layers are not rigid structures; the loosely bonded phospholipid molecules can move laterally across the surfaces of the membrane, and the interior is in a highly liquid state.
The experiments show that the speed of competition can be manipulated by altering how quickly key polarity proteins move between the membrane and the interior of the cell.
However, the ability of Fbxl7 to cause changes in the localization of Cindr and Ft indicates that it can regulate trafficking of proteins between the apical membrane and the interior of the cell in either direction, and the pathways that regulate the trafficking of these proteins and D might share common components.
As shown in Figure 1, Cyt-C expression were detected and presented as high electron-dense black dots, which were distributed in the exterior and interior membranes of mitochondria.
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