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All M. sylvestris individuals with a cumulative membership coefficient for the M. sylvestris gene pool >0.9 were considered to be pure M. sylvestris trees.
More than half of the cherry varieties showed the highest membership coefficient for a specific cluster (dark red), while the remaining showed admixture, primarily with the processing varieties.
A graphic representation of the estimated membership coefficient for each individual to each cluster at 1 ≤ K ≤ 5 is shown in Fig. 3.
The bar plots refer to the membership coefficient for each isolate organized according to 13 field populations under the assumption of three subpopulations.
Based on allele frequencies, we used this MCMC simulation to assign a membership coefficient for each individual to each K populations.
We regarded an individual as purebred when the average membership coefficient for the cluster corresponding to its mitochondrial haplotype was > 0.9 and as a hybrid when it was ≤ 0.9.
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Membership coefficients for individuals with posterior probabilities less than 0.05 were disregarded and proportionally assigned to the other cluster assignment coefficients.
A plot of individual membership coefficients for K = 19 reveals a high number of population clusters with average individual membership coefficients (i.e. posterior probabilities) greater than 0.9 (Figure 2, Table 2).
STRUCTURE plots for K>19 do not yield additional clusters with high membership coefficients for more exclusive sets of populations or individuals, but instead further divide already admixed sets of individuals (e.g. populations within M. murinus) into additional admixed cluster assignments.
Membership coefficients for the red cluster ranged from 0.617 to 0.816.
This matrix provides the estimated membership coefficients for each accession in each of the subgroups.
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