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The four isolates of Bacillus pumilus were found to consist of two different genotypes which grouped into two clusters each formed by two members (data not shown).
In contrast to Sirt3, PGC-1α did not alter the expression of other sirtuin members (data not shown).
For other organisms however, the expansion of this family originated from different members (data not shown), suggesting that NLRs of non-human origin have been lost during evolution.
Examinations of the length-rigid domain superfamilies showed that although ∼26% occur in multi-domain context and involve oligomeric interactions (Table S3), the type of domain neighbor is less varied with the same domain combinations reappearing across many members (data not shown).
Zebrafish also contain multiple putative TOX family members (data not shown).
As judged from completed genomes, many protists also encode Class I-like family members (data not shown).
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However, PRIMA1 expression was not detected in cell lines from either family member (data not shown).
It appears likely that the Group B members have evolved by gene duplication given their high sequence homology to their respective Group A member (data not shown).
However, most of these differences represented distinctions between the slender and stumpy forms, and 15 of the groups contained only a single member (data not shown).
We also observed, in genotype analysis of the initial 105 monkeys that lowering the sequencing coverage of any single monkey has very little impact on the accuracy of genotyping of pedigree members beyond the trios of which s/he is a member (data not shown).
However, cytoplasmic SV2 staining did not covary with two other SAE members: stonin2 (data not shown, ICQ 0.02±0.01, N = 0; p = 0>0.2) and AP180 (data not shown, ICQ 0.05±0.02, N = 8; p = 0>0.05), arguing against an association of the complete SAE with SVs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com