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The G/C content altered melting temperature values of amplicons but had no impact on HRM scores.
The equilibrium melting temperature values of TSC crystallites increased continuously with increasing the content of P d-2HB) relative to that of P d-2HB
The results reveal that these materials show density, enthalpy of fusion, and melting temperature values which are close to those corresponding to chain extended crystals.
The results show that the NCPE film prepared by hot-pressing method has smoother surface, higher interface stability, lower crystallization and melting temperature values than that prepared by solution-casting method.
Structure and crystallinity were followed by d.s.c., solid-state n.m.r. and attenuated total reflectance Fourier transform infra-red spectroscopy (ATR FTi.r).. Heat of fusion and melting temperature values ranged from 52 to 93 J g−1 and 228 to 242°C, respectively.
The protein melting temperature values were measured by the Rotor-Gene Q-Pure Detection software version 2.0.2 (Build 4).
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The specific melting temperature value of the real-time product was about 76.8°C.
Interestingly, these dual agonist proteins yielded only a single melting temperature value that was intermediate between that of their individual receptor agonist components, indicating that these chimeric molecules behave as a single domain protein during thermal denaturation.
We used cycle threshold (Ct) and melting temperature (Tm) values to discriminate amplified cDNA products from primer dimers (Fig. 1b, Additional file 1: Figure S3).
Near the melting temperature, the measured values of non-isothermal crystallization rate are close to those evaluated from isothermal crystallization, while at lower temperatures the difference between both quantities increases substantially.
It was expected, that the values of melting temperature decrease, bending angles and KD values clearly define the row of model DNA substrate properties such as Flu-dU-DNA >> nAnt ≈ Fap-dC-DNA.
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