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Interestingly, the cis form of Azo-py induced a more stable i-motif structure than did the trans form, as confirmed from circular dichroism spectra and melting temperature data.
A selected database of melting temperature data of ionic liquids available in the literature was constructed and used for determining the melting temperature of those substances for which homology was possible to be applied.
It was not possible to obtain reliable melting temperature data for SmTAL2.
Apparent melting temperature data are not shown for other TrEGI enzymes.
Apparent melting temperature data for Sc_TrEGI (with and without glutaminyl cyclase treatment) and Tr_TrEGI are shown in (Additional file 1: Figure S9).
PCR melting temperature data from LightCycler computer records before 2009 were also retrospectively reviewed to identify cases of EML pathogen infection.
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As in the case of mass loss, the PLA grade influenced the melting temperature profile during degradation (data shown in the Supporting Information).
Reactions were performed at 95°C for 5 min, and 95°C for 10 s, 61°C for 30s, 72°C for 10 s for 45 cycles, and a melting temperature cycle with constant fluorescence data acquisition from 65 to 95°C.
Thermal cycling conditions were 95°C for five min, then 40 cycles of 95°C for 10 sec, 60°C for 10 sec, and 72°C for 15 sec, followed by a melting temperature cycle, with constant fluorescence data acquisition from 65°C to 95°C.
The standard amplification protocol consisted of an initial denaturing step of 95°C for 30 s, followed by 45 cycles of 95°C for 5 s, 60°C for 10 s, 72°C for 15 s, and a melting temperature cycle with constant fluorescence data acquisition from 65°C to 95°C.
The approach is based on rigorous thermodynamics, using standard experimental thermodynamic data at the melting temperature of the pure compound and solubility measurements in different solvents at various temperatures.
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