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However, multiplexing obtained by HRM alone will be limited and probe-based melting technologies face a difficulty of probe design, and low resolution (temperature difference) of variants remains to be limited by using existing probe chemistries (DNA, LNA. etc).. To solve these problems, we adopted full base peptide nucleic acid (PNA) oligomer to probe-based melting point analysis.
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Newly designed Ti44Al6Nb1.0Cr2.0V0.15Y0.1B (at.%) alloy was prepared by vacuum consumable melting technology (melted two times), and then it was processed by cold crucible directional solidification (CCDS) technology at input power of 45 kW and a range of pulling velocities.
In this study selective laser melting technology was used for the first time to manufacture highly porous pure tantalum implants with fully interconnected open pores.
The objective was to analyze the influence of the manufacturing strategy on the internal structure and mechanical properties of the components manufactured by selective laser melting technology.
The prototype samples with a 135° alternate angle were fabricated of 316L stainless steel by selective laser melting technology and an experiment was carried out to test their kinematic performance.
This report describes the successful functional and esthetic reconstruction of the mandible using electron beam melting technology, an alternative technique for reconstruction of mandibles that did not undergo radiation therapy.
In this study, composite slabs with columnar-grained Fe-6.5 wt%Si alloy at the inner part and pure iron at double sides were prepared by the Bridgman zone melting technology under the melting temperature of 1485±5 °C and withdrawing velocity of 1 mm/min.
High resolution melting technology (HRM) was employed to analyse the SNP markers using a LightScanner instrument (Idaho Technology), following the protocols described by Montgomery et al. [ 74].
Initially developed for mutation scanning and genotyping studies [ 8- 12], high-resolution melting technology can be useful for the detection of methylation as well.
In contrast to DArT, SSR and SNP markers, InDel markers with moderate polymorphism differences can be amplified using regular PCR instruments and genotyped using an agarose gel electrophoresis system or HRM (high-resolution melting) technology.
For markers wri1, wri3 and sts638, genotypes were assayed using high-resolution melting technology [ 37] in a Roche LightCycler® 480 using conditions reported in Additional file 5. Melting curves were analysed using the gene scanning module of Roche LightCycler® software version 1.5.0.
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