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The results calculated by our simple hygrometer were compared with those by other hygrometers using experimental melting study data of arc basalts (see Figure 3 and Additional file 4: Table S2).
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The data in this report demonstrate, for the first time, a strong concordance between the effect of mismatches in probes attached to a three dimensional matrix of a microarray and solution-based, thermodynamic duplex melting studies.
In a set of 90 population study samples, none of the 26 known to belong to hg H [26] yielded detectable melt-peaks with singleplex 2706G ARMS-DCA under the experimental conditions used for the population study (data not shown).
Fig. 1 Workflow for extraction of melting point data.
The melting curve data were collected to check PCR specificity.
The RNAs were then melted in a CFX96 real-time PCR thermocyler (BioRad), and melting curve data points were taken every 0.5°C.
Manipulation of fluorescent melting curve data, including calculations of melting and annealing profiles and their associated analyses, were performed in Microsoft Excel (2003).
It was not possible to obtain reliable melting temperature data for SmTAL2.
Samples that yielded imbalanced melting peak data were called "Unknown" by the LightTyper software.
Unfortunately, correlations relating chemical structure directly to melting point data remain elusive.
The melting curve data were collected to check the PCR specificity.
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