Sentence examples for melting step was from inspiring English sources

A melting step was then performed with continuous fluorescence acquisition starting at 60°C with a rate of 1°C/5 s up to 99°C to determine the amplification specificity.

After the thermocycling reaction the melting step was performed with slow heating, starting at 55°C and with a rate of 0.5°C per 10 s, up to 95°C, with continuous measurement of fluorescence, allowing detection of possible non-specific products.

The melting step was performed using 30 acquisitions per °C.

A final melting step was performed from 65 °C to 95 °C with 1 °C increments every 5 s.

The addition of analyte causes a shift of the melting temperature to 59 °C, but only a single melting step is observed.

For the melting step, the temperature was raised from 75°C to 97°C with a ramp rate of 0.02°C/s and 25 acquisitions/°C.

After the PCR and melting steps, samples were loaded on 2% agarose gels to check whether amplifications were specific.

Before the high resolution melting step the products were heated to 95°C for 1 minute.

Although the amplification reaction of NASBA is isothermal, a single melting step prior to the amplification reaction is required to allow annealing of the primers to the target, which is not required in LAMP [35, 36].

The cycling parameters were: initial melting step at 95°C for 15 sec, and amplification at 95°C for 5 sec, then 60°C for 30 sec.

PCR conditions were initial melting step at 94°C for 5 min, 35 cycles each comprised of 94°C for 45 sec, exon specific annealing temperature for 1 min and 72°C for 1 min.