Sentence examples for melting step of from inspiring English sources

Samples were subjected to a melting step of 95°C for 10 min followed by 40 cycles of 15 s at 95°C and 1 min at 60°C.

The reaction continued with a single-cycle melting step of 95°C for 10 seconds, 67°C for 30 seconds and 95°C for 10 seconds, prior to cooling for 1 minute.

The cycling conditions were: 3 min 95 °C, 35 cycles of 5 °C95 °C, 30 °C64 °C and a melting step of 65 95 °C with a ramp rate of temperature increase of 0.1 °C with a hold of 2 s.

Cycling was performed using the following conditions: 95°C for 15 min, 45 cycles of 95°C for 30 sec, 50°C for 30 sec and 72°C for 60 sec and a melting step of 60-95°C 60-95°Cend of the thermat cycling.

The PCR profile included an initial melting step of 2 minutes at 95°C, followed by 35 cycles of 30 seconds at 95°C, 30 seconds at 60°C, 45 seconds at 72°C, and a final elongation step of 5 minutes at 72°C.

The PCR began with an initial melting step of 94°C for 3 min, followed by 20 cycles of 94°C for 30 s, annealing at 65°C for 30 s (decreasing 1°C per cycle), and extension at 72°C for 30 s.

PCR conditions consisted of an initial melting step for 5 min at 98°C followed by 30 cycles of 98°C for 30 s, 56°C for 45 s and 72°C for 45 s (40 cycles), a final extension step at 72°C for 5 min and a cooling phase to 14°C.

Two stages of decomposition are the key step of pyrolysis since they took up half or more of the reaction time; melting step consumed another half of reaction time in experiments when raw materials were heated up from ambient temperatures; and coke-like deposition appeared as a result of decomposition completion.

The thermocycling profile consisted of an initial melting step at 98°C for 30 sec, followed by 30 cycles of 98°C-6 sec; 60°C-20 sec; 72°C-15 72°C-15

The PCR profile for G172T polymorphism consisted of an initial melting step at 95 °C for 5 min, followed by 30 cycles of 95 °C for 30 s, 65 °C for 45 s and 72 °C for 50 s plus a final extension step of 72 °C for 10 min.

The second melting step corresponds to the denaturation of the MB analyte duplex.