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A PCR product was generated in a single reaction followed by melting point analysis.
This was followed by melting point analysis from 65°C to 95°C at a ramp rate of 0.2°C sec−1.
All the new compounds were fully characterized by NMR (1H, 13C, and 11B), FT-IR, UV vis, LC-MS spectroscopy, and melting point analysis and microanalysis.
Subsequent melting point analysis followed after 15 s at 95°C and 1 min at 60°C from 65°C to 95°C with an increment of 0.5°C for 15 s and plate read.
The melting point analysis revealed melting point decrease, but no significant sensitivity and signal loss in the presence of numerous (up to five) target-probe mismatches, indicating the capability of tolerating even more mutations.
At the end of each run melting point analysis was performed to validate specificity of PCR transcripts.
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Complex 1 was characterized by its melting point, elemental analysis, infrared spectroscopy, thermogravimetric analysis and single crystal X-ray diffraction.
The designed precursor 1 has been synthesized by a simple chemical reaction in high yield and characterized by its melting point, elemental analysis, FT-IR, mass spectrometry, single crystal X-ray analysis and thermal analysis (TGA/DTA).
thermogravimetric analysis, proton and carbon NMR (1H and 13C) spectroscopy, melting point, elemental analysis and polarimetry for its detail characterization, and assessment for industrial application potential.
The interaction parameters obtained from melting point depression analysis are −0.089 and −0.075 for PHB/PECH EO and PHBV/PECH EO blends, respectively.
The group-specific end-point and real-time PCR products could be differentiated according to species/genus and spoilage potential using restriction fragment length polymorphism (KpnI, XmnI, BssHII, ScaI) and melting point curve analysis, respectively.
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