Sentence examples for melting master from inspiring English sources

Sodium bisulfite-converted DNA (15 ng) was added to the PCR reaction mix, which consisted of high-resolution melting Master Mix (Roche Applied Science), 0.25 μM primers, and Mg2+ (2.5 mM).

Nested PCR were performed by using Light Cycler 480 (Roche Applied Science) in a total volume of 10 μl of the mixture containing 1 μl of diluted LR product, 1 × Light Cycle 480 High resolution melting master mix (Roche Applied Science) and 5 pmol of each primer.

Reaction mixtures contained 10 ng genomic DNA, 0.25µM of each primer, 3mM MgCl2 and 1× LightCycler 480 High Resolution Melting Master Mix (Roche Diagnostics, Meylan, France) containing Taq polymerase, nucleotides and the dye Resolight in a total volume of 10µl.

The reaction was performed in a 15 µl PCR mix containing 1X LightCycler® 480 High Resolution Melting Master Kit (Roche), 3.5 mM MgCl2, 0.5 µM of each primer and between 10 and 20 ng of DNA The amplification protocol consisted of a first denaturation step at 95°C [5 min], 45 cycles of denaturation at 95°C [10 s], annealing at 55°C [30 s], and extension at 72°C [30 s].

Amplification was performed on the ViiA7™ Real-Time PCR System Life Technologiess) using the LightCycler® 480 High Resolution Melting Master Mix (Roche Diagnostics).

DNA was amplified in a total volume of 10 μl, including 1 μl (5 30 ng) of genomic DNA, 0.2 µM of each primer, 4.2 mM MgCl 2, and 1× Roche High Resolution Melting Master kit mix.

"Game of Thrones" has always featured strong women: Catelyn Stark guided her son on the battlefield, Cersei Lannister craftily ended her husband, the former king, and Daenerys Targaryen melted slave masters with a single utterance.

She has yet, for example, to master that rolling, melting sashay of a walk.

The reaction mixture was prepared in a 20  μl final volume containing 1X HRMA Melt Doctor Master Mix (Applied Biosystems, Foster City, CA, USA) including a modified SYTO-9 as fluorescent DNA intercalating dye, 400 nℳ of each primer, 10 ng of genomic DNA and PCR grade water.

Components consisted of an acetate base layer, a double-sided adhesive layer for alignment, a cellulose wicking strip patterned with melted wax, a cellulose master mix pad, and a glass fiber pad for holding magnesium acetate.

Real-time PCR and melting curve analysis were done in a LightCycler 3.0 (Roche Diagnostics) using SYBR Green master mix (Roche Diagnostics).