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One minor drawback is the need to obtain a melting curve database to serve as a standard when identifying unknown samples [ 45].
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See Supplementary Figure 2 for melting curve analysis.
Finally, a melting curve analysis was performed.
A melting curve analysis followed.
Specificity was verified by melting curve analysis.
(B) melting curve analysis.
Products were verified by melting curve analysis.
Primer efficiency and melting curve analysis.
The melting curve analysis was conducted between 55°C-95°C 55°C-95°C
Figure 4 shows the normalized melting curve, difference plot and derivative melting curve of ABD6-1.
All primers were verified by melting curve analyses containing a single melt curve peak.
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