Sentence examples for melting curve data from inspiring English sources

The melting curve data were collected to check PCR specificity.

The melting curve data were collected to check the PCR specificity.

Quantitative RT-PCR melting curve data were collected to check the specificity of PCR.

The RNAs were then melted in a CFX96 real-time PCR thermocyler (BioRad), and melting curve data points were taken every 0.5°C.

Manipulation of fluorescent melting curve data, including calculations of melting and annealing profiles and their associated analyses, were performed in Microsoft Excel (2003).

The specificities of PCR products were analyzed by melting curve data and agarose gel electrophoresis to determine product size and to confirm that no by-products were formed.

The protocols were 1 cycle at 95 °C for 3 min, followed by 40 cycles at 95 °C for 15 s, annealing at the optimum temperatures for 30 s, 72 °C for 30 s and melting curves data were analyzed to monitor the purity of the PCR product.

We confirmed that a single target product was amplified by real-time PCR, based on melting curves (data not shown).

The products were then denatured at 95.0°C for 1 min and cooled to 65.0°C and kept for 1 min (melt curve data collection).

Low SG concentration may not only compromise PCR signal strength but also may make DNA melt curve data unreliable due to the so-called "dye redistribution" phenomenon [ 13].

It is desirable to have a relatively high threshold dye concentration so that PCR signals can be made more robust and DNA melt curve data more reliable (See discussion section).