Sentence examples for melting analysis were from inspiring English sources

PCR amplification and high-resolution melting analysis were performed on the Rotor-Gene Q (Qiagen).

The melting analysis was performed by increasing the temperature from 68°C to 82°C by 0.1°C/s.

The high-resolution melting analysis was performed using a LightScanner (Idaho Technology, USA).

A curve melting analysis was performed to confirm the amplification of a single PCR product.

At the end of the amplification cycle, a melting analysis was conducted to verify the specificity of the reaction.

High resolution melting analysis was subsequently carried out from 65 °C to 95 °C with a ramp rate of 0.02 °C/s.

High-resolution melting analysis was able to discriminate the p.Gly12Val (H441) and the p.Gly13Asp (HCT116) DNA from KRAS-WT DNA (PC-9).

High-resolution melting analysis was also performed with the LightCycler® 480 instrument (Roche Applied Science) using 96-well plates (Roche Applied Science).

Loci-spanning probe melt analysis was preformed by continuous signal acquisition between 38°C – 65°C at default ramp rate of 0.6°C/s and 5 acquisitions per °C.

Melting analysis was done from 72 to 95°C.

High Resolution Melting analysis is a well established, closed tube, rapid and high throughput technique.