Sentence examples for melting analysis was from inspiring English sources

A closed-tube RET genotyping assay using a saturating DNA dye, unlabeled probes, and amplicon high-resolution melting analysis was developed.

Additionally, a novel real-time PCR method with high resolution melting analysis was developed to target the 16S rRNA gene of both bee species, allowing their discrimination in different clusters.

High resolution melting analysis was performed at a ramp from 65°C to 90°C, increasing by 0.3°C each step, holding for 90 s prior to each melt, and also holding for 2 s after each melt.

The high-resolution melting analysis was performed using a LightScanner (Idaho Technology, USA).

A curve melting analysis was performed to confirm the amplification of a single PCR product.

The melting analysis was performed by increasing the temperature from 68°C to 82°C by 0.1°C/s.

PCR amplification and high-resolution melting analysis were performed on the Rotor-Gene Q (Qiagen).

Loci-spanning probe melt analysis was preformed by continuous signal acquisition between 38°C – 65°C at default ramp rate of 0.6°C/s and 5 acquisitions per °C.

High Resolution Melting analysis is a well established, closed tube, rapid and high throughput technique.

High-resolution melt analysis was used to evaluate 33 SNP markers across the diversity panel.

The sensitivity of detection by real-time RT-PCR coupled with probe-melting analysis was proved to be equal to that detected by conventional real-time RT-PCR.