Sentence examples for melting analysis of from inspiring English sources

High-resolution melting analysis of polymerase chain reaction products for mutation scanning, which began in the early 2000s, is based on monitoring of the fluorescence released during the melting of double-stranded DNA labeled with specifically developed saturation dye, such as LC-Green.

Figure 6B shows the ABD6-1 melting analysis of 4D7 progenies.

In 217 patients, tissue was available for high-resolution melting analysis of mutations in exon 15 of BRAF.

For 83 patients mutational status of the oncogene BRAF (V600E, GTG>GAG substitution in exon 15) was assessed by high-resolution melting analysis of genomic DNA on a Lightcycler 480 II platform (Roche, Mannheim, Germany; SYBR Green I/HRM Dye Protocol), in a modification of published protocols (Pichler et al, 2009).

Evaluating the T m and C t value for each target in each subsample Evaluating the T m and C t value for each target in each subsample The primary criterion in CoSYPS is the Tm value obtained upon melting analysis of the PCR amplification products.

The mutational status of the oncogene BRAF (V600E, GTG>GAG substitution in exon 15) was assessed by high-resolution melting analysis of genomic DNA on a Lightcycler 480 II platform (Roche, Mannheim; SYBR Green I/HRM Dye Protocol), in a modification of published protocols (Pichler et al, 2009).

The small insertion and deletion mutations that result from inefficient non-homologous end joining at this site are detected by high resolution melt analysis of whole flies and individual wings, allowing stable lines to be made within 1 month.

For example, restriction cut analysis following PCR amplification of the ITS1 [ 15, 17], high resolution melt analysis of the kDNA / PCR amplicon or the 7SL gene [ 12, 18, 19].

At the end of the PCR, samples were subjected to a melting analysis to confirm specificity of the amplicon.

A subsequent fluorescence curve melting analysis (FCMA) of the PCR products revealed clearly distinguishable single or double melting peaks for four aptamers which are shown in color in Fig 2a.

In this study, fluorescence intensity was measured at melting analysis step instead of amplification step because of its time-consuming disadvantage.