Sentence examples for melting analysis in from inspiring English sources

A practical and more flexible alternative may be available using amplicon melting analysis in conjunction with real-time PCR [ 17].

For each primer pair, the amplicons from Excalibur and Kukri were examined for polymorphism using electrophoresis on agarose gels, high-resolution melting analysis in a Roche LightCycler® 480 and/or sequencing.

We compared the COLD-PCR melting curve assay to regular PCR melting analysis in a cohort of 61 FFPE colorectal cancer specimens which included 14 blinded samples that had KRAS testing done previously at outside institutions.

Using methylation-specific high-resolution melting analysis in a set consisting of 133 colorectal cancer samples, we were able to confirm an altered CpG site in TMEM25 in 69.2% (92/133) tumours analysed.

The present study demonstrates the utility of TSP for the rapid development of robust endpoint SNP genotyping assays based on allele-specific PCR and high resolution melt analysis in cultivated barley (Hordeum vulgare L ., an agriculturally important crop with a 5,300 Mb diploid genome (about 2× size of human genome).

We evaluated the efficiency of high-resolution melting analysis (HRMA) in identifying KRAS-mutant (MUT) tumours.

Amplification specificity was confirmed by melting analysis or, in the case of EFα1 primers, by agarose gel electrophoresis.

In addition, HRM is more rapid as the melting analysis is performed in all wells simultaneously whereas DHPLC involves the removal of the PCR product (in itself problematical because of the PCR contamination issue) and then the sequential analysis of each sample.

In 217 patients, tissue was available for high-resolution melting analysis of mutations in exon 15 of BRAF.

We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.

The great advantage of MS-HRM and dMS-HRM is that they are in-tube methodologies that are rapidly performed and do not need post-PCR processing as the melting analysis is carried out in the same reaction vessel as the amplification.