Sentence examples for melting analysis data from inspiring English sources

High-resolution melting analysis data were analysed with the 7500 fast Real-Time HRMA Software v 2.0.1 (Applied Biosystems) and evaluated by a molecular geneticist (UM) and molecular pathologist (GT).

However, scoring the samples of unknown mutation status is not always straightforward as visual inspection of both the derivative of the raw data (melt curve analysis) and the normalized HRM and difference graphs (high resolution melting analysis) may be needed for correct interpretation of the melting data.

The samples were scored manually by visual inspection of the derivative of the raw data (melt curve analysis) and the normalized HRM and difference graphs (high resolution melting analysis).

Primer pairs for high resolution melting analysis.

by high resolution melting analysis [ 28].

This approach is melting analysis rather then the high resolution melting (HRM) we have used here.

Figure 6B shows the ABD6-1 melting analysis of 4D7 progenies.

After PCR amplification and melting curve analysis, data were analysed by the LightCycler 480 Gene Scanning software V.2.0 (Roche Diagnostics, Germany).

High specificity of PCR is demonstrated by agarose gel electrophoresis and melting curve analysis (data not shown).

The RT-PCR experiments examined expression patterns of individual genes, as evidenced by the single peak in the melting curve analysis (data not shown), while hybridization-based microarrays do not necessarily distinguish between gene family members.

The undiluted G12 D and G13 D FFPE samples both had ~ 25% KRAS mutant allele based on a visual estimate of tumor nuclear content and on the mutant:WT peak height ratios in regular PCR melting curve analysis (data not shown).