Sentence examples for melting analysis and from inspiring English sources

We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.

The thermal stabilities of the junctions were assessed by UV melting analysis and formation of constructs was confirmed by gel electrophoresis.

The specificity of each amplification was checked by melting analysis and agarose gel electrophoresis of the amplified products.

High resolution melting analysis and DNA sequencing: Our laboratory routinely uses qPCR-HRM as a pre-screening tool, which prevents useless sequencing of wild-type DNA.

Allelic variation has been detected in coding sequences by several screening methods, including DNA nuclease assays, high-resolution melting analysis and next-generation sequencing.

High resolution melting analysis and confirmatory sequencing was used to determine the presence of somatic mutations in PIK3CA (exon 9 and 20), AKT1 (exon 4), KRAS (exon 2) and BRAF (exon 15) genes in 57 familial MBCs.

A subset of these putative SNPs was validated using high-resolution melt analysis and Sanger resequencing to genotype independent samples of kokanee and anadromous sockeye salmon.

For example, Bassett et al. (2013) and Gratz et al. (2013a) adopted high-resolution melting analysis (HRMA) and the surveyor kit (Transgenomic, Inc), respectively.

Recently, HRM [ 15] and DNA, LNA probe melting analysis have been introduced and used for genotyping studies [ 16].

Primarily, these studies have been performed using UV melting analysis, circular dichroism (CD) measurements, and molecular modeling.

Monis et al. (2005) showed that SYTO 9 was suitable for fluorescence monitoring of PCR and standard melting analysis [ 57].