Sentence examples for melting analyses of from inspiring English sources

To systematically analyze the effects of asymmetric PCR on LightCycler melting analyses of four different allelic-discrimination systems and to reduce an inconsistent non-specific melting peak observed during factor V Leiden genotyping.

UV-melting analyses of DNA strands indicated that the αPLL copolymers showed the highest stabilization efficacy toward poly dA)·poly dT) duplex and poly dA)·2poly dT) triplex without affecting reversibility of inter DNA association.

Accuracy of Ct-values was assured via melting curve analyses of every analyzed reaction.

The specificity of the expected RT-PCR products was determined based on melting curve analyses of reactions with glioma cDNA and human cDNA libraries.

The amplification was carried out under the following conditions; 50°C for 2 min followed by an initial denaturation step at 95°C for 2 min, 40 cycles at 95°C for 15 s, 60°C for 30 s, and 72°C for 30 s. Non Template Controls (NTCs) and melting curve analyses of amplicons were analysed for all experiments.

The conditions used for real-time PCR are as follows: 15 min at 95°C, followed by 35 cycles of 94°C for 30 s, 58°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. Quantitative and specificity (melting curve) analyses of amplified PCR products were carried out using associated software in the Opticon system (Opticon monitor3) by following the manufacture's protocol.

He studies the role of water in differentiation and eruption of arc basaltic magmas by conducting hydrous melting experiments and analyses of melt inclusions.

Due to High Resolution Melting Analyses (A segregation technique using Melting point of DNA) the Rotor Gene 6000 Corbettt) 20 samples were designated to be sequenced only in forward direction.

The C677T Alaa → Val) and A1298C (Glu → Ala) variants were analysed simultaneously by means of melting curve analyses on LightCycler (Roche), based on the fluorescence resonance energy transfer (FRET) principle (see Figure 2 for a typical example of analysis).

Melt curve analyses of all real-time PCR products were performed and shown to produce the sole DNA duplex.

Melt curve analyses of all real-time PCR products were performed and shown to produce a single-DNA duplex.