The existing melting algorithms typically produce melting profiles of some numerical quantity for each sequence position.
DHPLC was carried out at the recommended melt temperature for each exon (Table 1) as determined by the Stanford melt algorithm [ 34, 35].
DHPLC was carried out at both the recommended melt tempe-rature as determined by the Stanford melt algorithm (http://insertion.stanford.edu/melt.html) and 2 above the recommended temperature.
In particular, we analysed each PCR fragment at all the temperatures recommended by the DHPLC melt algorithm and under these conditions DHPLC has been reported to have a sensitivity of 99.4% [ 40].
This group developed a probe-selecting algorithm incorporating U, melting temperature (Tm), and synthesis cycle number with sequence-specific filters.
The DNA melting data were analyzed by LC480 Gene Scanning software (version 1.5) which, after data normalization and temperature-shifting, grouped cultivars with similar melting patterns using a proprietary algorithm.
Each amplicon was analysed to ensure that it contained only a single melting domain using the Poland algorithm [ 36]. # M13 sequences (not shown) are attached to all sequencing primers.
We verified our algorithm by computing the melting temperature for RNA pairs available in the literature and the equilibrium concentration for OxyS-fhlA complex.
For example, concepts like boiling point, melting point, basis set, entropy, enthalpy, methodology, algorithm, etc., are not included in the CML Schema.
However, it was not obvious that this problem has time complexity O(N log N), which is the same as computing melting profiles with the Poland-Fixman-Freire algorithm [ 32].
