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The timing and nature of lake ice growth and melt were recorded.
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In 2012, melt was recorded at the very top of the ice sheet.
A clear increase in temperature fluctuations indicating timing of snow melt was recorded by all temperature loggers (see additional file 1).
Thermal melts were recorded from 4 to 90 °C at 222 nm.
To confirm single amplicons, melt curves were recorded after final cycle heating from 65 °C to 95 °C with an increment of 0.5 °C per step.
Melt viscosities were recorded on a UDS 200 Rheometer (Physica©).
CD temperature melt data were recorded for SYCP3Core (0.15 mg/ml in 10 mM NaH2PO4 pH 7.5, 150 mM NaF) and SYCP3FL (0.51 mg/ml in 20 mM Hepes pH 7.5, 500 mM KCl), at 5°C increments between 5 and 95°C, with 1°C per minute ramping rate, 0.5°C deadband, 30 s incubation time, 1 nm slit width and 1 s averaging time.
Melting points were recorded on an Electrothermal melting point apparatus and are uncorrected.
Melting points were recorded on a Griffin melting point apparatus and are reported uncorrected.
Melting points were recorded with an Optimelt automated melting point system with a heating rate of 1 °C min 1 (70%% onset point and 10%% clear point).
Melting points were recorded on a Barnstead Electrothermal 9100BZ melting point apparatus (St . Louis MO, USA).
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