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But it now appears that the estimate about Himalayan glacial melt was based on a decade-old interview of one climate scientist in a science magazine, The New Scientist, and that hard scientific evidence to support that figure is lacking.
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The method of rapid quenching of the melt is based upon the rapid heat transfer from the melt to a cooling substrate of good heat conductivity.
A statement that the Himalayan glaciers were "very likely" to melt by 2035 was based on "poorly substantiated" estimates, IPCC said.
Melt curve analysis was based on temperature ramping from 55° to 95° in 0.5° increments over 5 min. PCR master mix included 15 microliters 2x GoTaq Green (Promega), 11.65 μL of water, 2.5 μL of cDNA and 0.85 μL of primer (10 μM forward and reverse).
The melting detection in this study was based on the disappearance of the X-ray diffraction peaks which were the same as those in previous melting studies (e.g., Kamada et al. 2010, 2012; Terasaki et al. 2011).
The initial selection of temperatures for real-time LCR cycling steps was based on melting simulations for each candidate LCR oligonucleotide and LCR product, with their respective complements.
However, this modeling was based on a melting regime obtained by compiling the results of melting experiments for a variety of basalts with and without H2O.
High Resolution DNA Melting curve analysis (HRM) is based on DNA melting in the presence of saturating DNA binding dyes.
High-resolution melting curve analysis (HRM) is based on the melting behavior of relatively short, double-stranded DNA fragments and is a fast and reliable post-PCR method to detect mutations like single-nucleotide polymorphisms (SNPs) or indels.
The play, which opens on March 13 at the McGinn/Cazale Theater in a production by the Melting Pot Theater Company, is based on the infamous Tuskegee Syphilis Experiment, a government-backed study in which hundreds of African-American men infected with syphilis were left untreated.
Melting temperature (Tm) calculations are based on thermodynamic models that use solution parameters and an assumption that reactions have reached equilibrium.
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