Sentence examples for melt analysis of from inspiring English sources

The small insertion and deletion mutations that result from inefficient non-homologous end joining at this site are detected by high resolution melt analysis of whole flies and individual wings, allowing stable lines to be made within 1 month.

For example, restriction cut analysis following PCR amplification of the ITS1 [ 15, 17], high resolution melt analysis of the kDNA / PCR amplicon or the 7SL gene [ 12, 18, 19].

Droplet Array Platform for High-Resolution Melt Analysis of DNA Methylation Density.

We screened for mutations in MAP2K4 using High Resolution Melt analysis of 149 primary ovarian tumors and methylation at the promoter using Methylation-Specific Single-Stranded Conformation Polymorphism analysis of 39 tumors.

Hanson & Ballantyne report in this article a novel use for High Resolution Melt analysis of RNA samples in order to identify single or mixed human body fluids (and tissues), namely vaginal secretions, skin, blood, menstrual blood, saliva, or semen using a series of molecular biomarkers: IL19, IL1F7 or CCL27, ALAS2, MMP10, HTN3, and TGM4, respectively.

Melt analysis of amplicons formed at the end of the run was generally found to be a good indicator of outcome but we would still recommend gel electrophoresis to confirm correct product size as occasionally negative samples which generate a "ladder" of non-specific products are encountered and these can complicate interpretation of the melt profiles.

Figure 6B shows the ABD6-1 melting analysis of 4D7 progenies.

In 217 patients, tissue was available for high-resolution melting analysis of mutations in exon 15 of BRAF.

For 83 patients mutational status of the oncogene BRAF (V600E, GTG>GAG substitution in exon 15) was assessed by high-resolution melting analysis of genomic DNA on a Lightcycler 480 II platform (Roche, Mannheim, Germany; SYBR Green I/HRM Dye Protocol), in a modification of published protocols (Pichler et al, 2009).

The mutational status of the oncogene BRAF (V600E, GTG>GAG substitution in exon 15) was assessed by high-resolution melting analysis of genomic DNA on a Lightcycler 480 II platform (Roche, Mannheim; SYBR Green I/HRM Dye Protocol), in a modification of published protocols (Pichler et al, 2009).

For 83 patients, the mutational status of the genes KRAS (codon 12 and 13 in exon 2) was analysed by high-resolution melting analysis of genomic DNA on a LightCycler 480 II platform (Roche; SYBR Green I/HRM Dye Protocol) as described previously in detail (Nitsche et al, 2012).