Sentence examples for melt analysis and from inspiring English sources

A subset of these putative SNPs was validated using high-resolution melt analysis and Sanger resequencing to genotype independent samples of kokanee and anadromous sockeye salmon.

Genomic DNA from the treated embryos was analysed for mutations in the mutated genes using High Resolution Melt analysis and confirmed by Sanger sequencing.

We also describe a simple and highly sensitive method of detecting mutations in the target gene by high-resolution melt analysis and discuss how the new technology enables the study of gene function.

Through amplicon melt analysis and direct sequencing of the 235-bp PCR-amplified fragment, the region corresponded to the expected specific sequence within the D1/D2 LSU rDNA domain, according to Hosoi-Tanabe and Sako (2005).

We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.

The thermal stabilities of the junctions were assessed by UV melting analysis and formation of constructs was confirmed by gel electrophoresis.

The specificity of each amplification was checked by melting analysis and agarose gel electrophoresis of the amplified products.

Allelic variation has been detected in coding sequences by several screening methods, including DNA nuclease assays, high-resolution melting analysis and next-generation sequencing.

High resolution melting analysis and DNA sequencing: Our laboratory routinely uses qPCR-HRM as a pre-screening tool, which prevents useless sequencing of wild-type DNA.

For example, Bassett et al. (2013) and Gratz et al. (2013a) adopted high-resolution melting analysis (HRMA) and the surveyor kit (Transgenomic, Inc), respectively.

Recently, HRM [ 15] and DNA, LNA probe melting analysis have been introduced and used for genotyping studies [ 16].