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Biomass was mineralized in the microwave decomposition equipment (Milestone 1200 Mega) using nitric acid and hydrogen peroxide.
Evolutionary distances were derived from sequence-pair dissimilarities calculated as implemented in MEGA, using Kimura's DNA substitution model [Kimura 1980].
The evolutionary distances were derived from the sequence-pair dissimilarities, calculated as implemented in MEGA using the DNA substitution model reported by Kimura (1980).
Neighbour-Joining population trees were calculated in MEGA using these pair-wise FST-values.
Standard errors of DXY and DA were calculated in MEGA using the Bootstrap procedure with 1000 replicates.
Phylogenetic reconstruction was performed in MEGA, using the neighbor-joining method.
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The phylogenetic tree was constructed with software MEGA 7.01 using the neighbor-joining method.
For the phylogenetic analysis, tree constructions were done with the software MEGA 7.0 using the neighbor-joining method (Hall 2013).
Phylogenetic analysis was performed by version 4.0 of MEGA software using a neighbor-joining algorithm of cauterization.
The NJ analysis was implemented in MEGA 4 [64] using the observed number of changes and 1,000 pseudo-replicated.
The NJ analysis with the bootstrap test was performed in MEGA 4.0 [55] using pairwise deletion for gaps/missing data.
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