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To generate the pooled experimental BSE sample for intra cranial inoculations, rostral medulla from a classical (C-type) Canadian BSE field case was homogenized in phosphate buffered saline (PBS) {2.7 mM KCl; 1.5 mM K(PO4); 8.1 mM Na2(PO4); 137 mM NaCl; pH: 7.4±0.2} to a final concentration of 10%w/v using a MediFAST/Prypcon system.
By comparison, GFAP staining in the cerebellar medulla from a normal 12-month-old Beagle was much less intense and was more diffuse.
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A section of the medulla oblongata from a BSE-infected cow was included to validate the procedure with every run.
Immunoblotting of caudal medulla from the eight pre-1975 cattlengavecattle gave a uniform molecular profile for all animals in the group.
A perhaps more interesting finding holding potential diagnostic implications comes from the observation that all VHL-related PCC, but not matching adrenal medulla from the same patients, displayed strong membranous CaIX staining, a peculiar pattern that was not replicated in other cases, where a granular cytoplasmic staining was seen.
The ability of mammals to produce urine hyperosmotic to plasma requires the generation of a gradient of increasing osmolality along the medulla from the corticomedullary junction to the papilla tip.
The caudal medulla from the remaining two steers from the post-1990 grouPG152/02/02 and PG512/02) gave a similar result to the other five animals but the unglycosylated protein band had a lower molecular mass.
It is worth noting, however, that there is precedent for changes in AC6 content in IMCD – Hoffert and colleagues have reported a 5-fold decrease in AC6 protein abundance in inner medulla from Brattleboro rats given dDAVP as compared to saline alone [ 11].
He, W. et al. Sirt1 activation protects the mouse renal medulla from oxidative injury.
These cells migrate to the adrenal medulla from the embryonic neural crest and represent specialized neural tissue.
A total of 320 digital micrographs of Periodic acid-Schiff (PAS) – stained kidney medulla from 16 Wistar albino mice (20 per animal), were analyzed using National Institutes of Health ImageJ software (NIH, Bethesda, MD) and its plugins.
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