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For statistic analysis, TER values of 16HBE cells after exposure to different acidification culture mediums were normalized by TER of 16HBE cells after exposure to pH 7.4 culture medium.
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Oxygen consumption and medium acidification values were normalized by protein content.
The marked increases in BACE1 mRNA and protein levels following medium-dose Ang II treatment were normalized by losartan(p>0.05), implying that these alterations were associated with central Ang II stimulation.
Raw data were normalized by quantile normalization.
Stimulated samples were normalized to the corresponding medium-only control.
Individual spectral profiles were normalized to the control medium to account for possible impact of variations in the culture conditions.
CFU of C. neoformans in conditioned media (from differentiated or undifferentiated HL-60) were normalized with CFU of C. neoformans in fresh medium.
eCFP ratios were normalized against cells to which 10 μl M9 medium had been added.
Absolute fluorescence values were normalized to the background values of the BG11 medium, and the wild type was used as fluorescence baseline.
We analyzed resazurin reduction in cell culture medium fluorimetrically (λex = 530 nm, λem = 590 nm), and values were normalized to untreated controls.
The data were normalized with that of a gene encoding clathrin adaptor complexes medium subunit (CAC; SGN-U314153) as a reference [ 62] (Additional File 2).
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