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At last the mediums were incubated for 24 h at 36 °C and 1 week at 25 °C for bacteria and fungi, respectively.
Then the cultured mediums were incubated at 35 to 37°C in an aerobic environment for 18 to 24 hours.
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For gene expression studies 1×10 CD4+ T cells in 3 ml RPMI 1640 medium were incubated for 4h at 37°C (5% CO2 incubator).
The trays containing Mueller Hinton broth were incubated at 35 °C for 18 20 h while the trays containing RPMI-1640 medium were incubated at 35 °C for 46 50 h.
Candida albicans monoculture, macrophage monoculture, and complete culture medium were incubated for 3 h and the proteins as a control were extracted, reduced, alkylated, and digested with trypsin.
The 250-mL Erlenmeyer flasks containing 100 mL of a mineral medium were incubated for 48 h at 30 °C in a rotary shaker at 200 rpm.
Briefly, 5 × 106 LNCaP cells in a 320-μL RPMI 1640 medium were incubated with 68Ga]6 or 68Ga]7 (25 nM final concentration), respectively, for 45 min at 37°C.
Cells in RPMI 1640 medium were incubated with C60 fullerene (16 μM) or HL (2.5, 5, and 10 μM) separately and together during 24, 48, and 72 h.
The experiment group with 100 ul suspension and control group with equal volume of DMEM complete medium were incubated for 24 h and were further incubated for 4 h after the CCK-8 was added to the incubator.
In 6-well plates, fully differentiated 3T3-L1 adinocytes in 3 mL growth medium were incubated with 1 μM DXM for 48 h, with or without the addition of 20 or 10 μM of 1 or 20 μM of rosiglitazone.
Cells (105 CFU/ml in broth agar medium) were incubated at 37°C with agitation (240 rpm/min) with rhLF solution (final concentration of 0.5 mg/ml, 2 mg/ml, or 5 mg/ml in broth agar medium).
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