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We then tested different solvent mediums, namely ethanol, DMSO and cyclodextrine for the hormone preparation and found little differences between the three mediums (data not shown).
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Intracellular expressed β-glucosidase activity was not detected in the medium (data not shown).
However, higher aeration rate resulted in more intense evaporation and convection of culture medium (data not presented).
Interestingly, when TKI basal salts were omitted from the 5% molasses medium, TS concentration decreased by 96% compared to cultivation in the original TKI medium (data not shown).
Expression of the genes was confirmed by RT-PCR of RNA isolated from fungi cultivated on microcrystalline cellulose medium (data not shown).
They were clearly different from H. seropedicae (under phase contrast microscopy) in cell shape and colony form when grown in JNFb solid medium (data not shown).
Also, the addition of bioactive glass has reduced contraction of the PLGA/collagen scaffold in contact with the culture medium (data not shown).
Xylosidase activity in YKX1 was higher than that in Y-94 grown in PD medium (data not shown), while activity of YKX1 was much higher than that of Y-94 in medium with 4%Solcaflock (Figure 3).
Both TCs + and TCs- cultured for 14 days in adipogenic medium showed no appreciable intracellular lipid vacuole production in comparison with cells maintained in non-inductive medium (data not shown).
The % conversion to racemic-β-citronellyl acetate in 4 h at 40°C increased from 80%to95%5% when 0.75% water (v/v) was added to the reaction medium (data not shown in the form of figure/table).
The shake flask cultivation of C. curvatus and C. podzolicus CPOH4 in mineral salt medium (data not shown) resulted in a decrease of the pH value from 5.0 to 2.0 within 60 hours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com