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In the case of phagocytosis experiments, cells were incubated in a SorC or HL5 medium-filled Petri dish, the bottom of which was made out of a glass coverslip (MatTek).
The coated filters were placed in Boyden chambers, forming two medium-filled compartments separated by such microporous membrane.
After 8 days, dead cells and conditioning media were removed, cells were trypsinized and a 10% aliquot was plated in fresh medium-filled six-well plates.
The Boyden chamber assay used for the analysis of tumor cell migration is based on a chamber with two medium-filled compartments.
Progressive motile spermatozoa migrate from the ejaculate in the outer well (3 ml) to concentrate in the medium-filled (BM1Medium, Eurobio, Courtaboeuf, France) inner well, using a capillary bridge created by the overlying U-ring.
Due to differences in viscosity, when the less viscous medium flows through the channel, it pushes through the more viscous hydrogel solution in a finger-like pattern leaving behind a medium-filled lumen in the hydrogel.
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Afterwards the monocytes or medium (filled grey) were added to untreated or BT-061 decorated T cells.
An internal natural convective flow in a porous medium filled closed square cavity is studied in the presence of thermal nonequilibrium between the fluid and solid phases.
As far as the molecular dynamics is concerned, highly filled systems (> 80 phr carbon black) behave differently from low and medium filled systems (< 80 phr).
(E) Purified CD3+ T cells were treated with BT-061 (as in B, black line) or with medium (filled grey) and then incubated for 18 h at 37 °C (upper row).
The analytical method applied for comparison uses the additional algebraic formulas derived for the homogenized tensor for a medium filled with spherical inclusions, while the FEM-based technique employs the polynomial response functions recovered from the Weighted Least-Squares Method.
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