Sentence examples for medium yep from inspiring English sources
When arrest was complete, cells were released into rich medium (YEP) with 2% raffinose lacking pheromone and containing 2% galactose, 1% DMSO and 8 mM methionine (to repress Cdc20 production).
Cells were grown aerobically at 30°C with constant shaking in rich liquid medium (YEP, 10 g/L Yeast Extract, 20 g/L Bacto Peptone) or in minimal synthetic liquid medium (SM, 1.7 g/L yeast nitrogen base, 5 g/L ammonium sulphate, and 20 mg/L uracil) supplemented with glucose or glycerol (2% each) as carbon sources.
Hiroshi Lockheimer, Google's SVP for Android, Chrome OS and Chromecast, writes on Medium today (yep, Medium and not Blogspot) that the team decided it wanted to release the preview earlier in order to get more feedback from developers earlier in the process and get the final N release into the hands of device manufacturers this summer.
Agrobacterium cells containing split YFP fusion constructs were grown in liquid yeast extract peptone (YEP) medium supplemented with antibiotics (spectinomycin 50 μg/ml and Rifampicin 50 μg/ml).
Cells were grown in YEP medium (1% yeast extract, 2% bactopeptone, 50 mg/l adenine) supplemented with 2% glucose (YEPD) or 2% raffinose (YEP+raf) or 2% raffinose and 2% galactose (YEP+raf+gal).
Agrobacteria carrying different constructs were grown overnight in YEP medium (50 μg/mL rifampicin, 50 μg/mL kanamycin and 25 μg/mL gentamicin), collected by centrifugation and resuspended to OD600 of 0.8 in infiltration buffer (10 mM MgCl2, 10 mM MES, 200 μM acetosyringone, pH5.7).
Cells grown in YEP lactate 3% medium at 28°C to a concentration of 5 × 10 cells/ml were arrested with nocodazole (20 μg/ml).
Strains AM5708 (BRN1-6HA ), AM10859 (BRN1-6HA pGAL-SGO1 ) were pre-cultured in YEP + R + Ade medium before supplementing with nocodazole and galactose (2 hr) for 3 hr and then harvesting for anti-HA ChIP.
Cells carrying BRN1-6HA and that were otherwise wild type (AM5708) or carrying pGAL-SGO1 (AM10859) integrated an ectopic locus, were arrested in G1 in rich medium containing raffinose and adenine using alpha factor (YEP + R + A).
Strains with the CDC20 gene under control of the MET3 promoter were grown in minimal medium lacking methionine and arrested in mitosis in YEP supplemented with 2 mM methionine.
