Sentence examples for medium without virus from inspiring English sources

Fig. 2 mRNA expression of β-actin and β(1) integrin in LMH cells cultured in normal growth medium (GM), inoculation medium without virus (IM), or infection medium six or 24 hpi with H8N4 virus Fig. 3 Vector uptake by chicken LMH cells assessed at two and 24 h post invasion with the anti-AIV/scramble vector tagged with RFP.

One well on each plate received 0.5 ml of medium without virus as a negative control.

HUVECs were incubated with 5 multiples of infection (MOI) of either Ad.ecSOD or Ad.ecSOD-ΔHBD or Ad.LacZ (control) in 5% FBS containing culture medium for 24 hr, followed by incubation in 0.5% FBS containing culture medium without virus for 24 hr before experiments, as we described previously [20].

Like in case of the infection, the growth medium was withdrawn and new medium (virus medium without virus) was added.

Viruses were diluted in Minimum Essential Medium (MEM) containing 0.3% BSA, and the same medium without virus was used for mock infections.

HPASMCs were infected for 2 hours with the above adenovirus using a multiplicity of infection (MOI) of 100, washed, and incubated in serum-free medium without virus for at least 24 hours before drugs challenge.

The negative control wells contained cells with maintenance medium only without virus and without treatment.

*Mock, controls treated with serum from healthy (no dengue infection) donors; virus, virus plus diluent; medium, serum and diluent without virus; SI, subgenotype I; SII, subgenotype II.

Cells were then washed with PBS and treated with 100 µl of SFM (same medium used for infections but without virus) containing 50 µM RBV 1% of which was [3H]RBV (ViTRax, Placentia, CA, cat. no. VT193, specific activity 5 Ci/mmol) for 15 minutes in a 5% CO2 atmosphere at 37°C.

For the long-term accumulation of RBV, cells (in triplicates) on 12-well plates were washed with PBS and treated with 275 µl of SFM (same medium used for infections but without virus) containing the same concentration of RBV/[3H]RBV (in the absence of NBMPR) as above but incubated for 1 h, 16 h or 24 h.

Two hundred microliters of maintenance medium were added to the negative control wells (cells without virus or treatment).