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Inoculum was prepared by growing the cells overnight in 1 L shake flasks containing 100 mL defined mineral medium with vitamins (Verduyn et al. [1992]) with glucose as carbon source (20 g.L−1) buffered with phthalate buffer (50 mM, pH 5.0).
Inoculum was prepared by growing the cells overnight in 100 mL shake flasks containing 20 mL defined mineral medium with vitamins (Verduyn et al. [1992]) with glucose as carbon source (20 g.L−1) buffered with phthalate buffer (50 mM, pH 5.0).
Micro-Tina tomato seeds were sterilized and sown on Murashige and Skoog (MS) medium with vitamins.
Fresh onion epidermal peels were transferred to MS medium with vitamins (0.5% MS Salts with vitamins, 0.7% Agar, pH 5.7) less than one hour before bombardment.
The culture medium used was a defined mineral medium, with vitamins prepared as described by Verduyn et al. [ 40].
For seedlings growing on plates, surface-sterilized Arabidopsis seeds were grown on Murashige and Skoog basal medium with vitamins (MS) (Phytotechlab, http://www.phytotechlab.com/) except where noted.
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Dermal fibroblasts cultured on β-TCP scaffolds in chemically-defined medium with vitamin D3 exhibited up-regulated gene and protein expression compared to cells cultured on OPLA scaffolds.
These results suggest that Vitoss (β-TCP) scaffolds seeded with dermal fibroblasts and maintained in chemically-defined medium with vitamin D3 are better suited for bone tissue engineering applications than Drilac (OPLA) foams.
They grew 43 types of cancer cells and five strains of normal cells on a medium with vitamin C. For 75% of the tumor types, less than 10 millimoles of the vitamin killed about half of the cells while sparing normal cells.
To prepare serum-supplemented medium foetal bovine serum (Hyclone/GE Healthcare, South Logan, Utah) was either added directly to filter sterilised HL5 medium supplemented with vitamins and microelements (HL5VME, Formedium), or first diluted to 10% vol/vol in Milli-Q water then used to dissolve powdered HL5VME before 0.2 μm filter sterilisation.
Sterilized seeds were then directly sown on MS/G plates consisting of ½ Murashige & Skoog (MS) basal medium supplemented with vitamins (Plantmedia, www.plantdemia.com), 1% (w/v) sucrose, 0.6% (w/v) phytoagar (Plantmedia), pH adjusted to 5.7 with 1N KOH, and with or without ABA.
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